Regulatory

Part:BBa_K4040033

Designed by: Tong Yin   Group: iGEM21_NMU_China   (2021-09-29)


UAS-pSV40

This part (UAS-pSV40 ) is a mammalian synthetic promoter (SynPro) based on Sv40 promoter (BBa_K2446052) and 4*GAL4 binding sites from UAS-Gal4 Promoter MammoBlock(BBa_K511003 MIT2011) . We inset the 4*GAL4 binding sites before the Sv40 promoter. Sv40 promoter is a constitutive expression promoter from the simian vacuolating virus 40. The GAL4DBD can bind to the 4*GAL4 binding sites specifically and the KRAB domain can repress the expression of UAS SV40 promoter. The corresponding SynTF of UAS-pSV40 is ZF_GAl4_KRAB (BBa_K2446037).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None